This protocol is generic in nature and is intended to provide relevant guidelines for the preparation of nanoparticle dispersions in liquid media by the application of ultrasonic energy (a process referred to here as sonication). It applies specifically to source engineered nanoscale materials (ENM) obtained in dry powder form, but can be extended to the treatment of previously suspended ENMs (e.g., for purposes of re-dispersion).
For a more in depth discussion of specific parameters addressed in this protocol, the reader is advised to consult reference . For guidelines on reporting relevant conditions and critical parameters relating to the ultrasonic dispersion of ENMs, refer to . For additional relevant and general considerations on the application of ultrasound, the reader is urged to consult the references [3-10].