Theme 3: Ecosystem Functional Responses to Nanomaterials

13. Trophic Transfer of Nanomaterials

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2011

12. Research at Multiple Scales and Levels of Complexity

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2011

3. Mesocosm Shared Facility

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2011

4. Preliminary Mesocosm Results

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2011

Mesocosm Construction

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2009
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CEINT/ NIST Protocol for Preparation of Nanoparticle Dispersions from Powdered Material Using Ultrasonic Disruption, Version 1.1

This protocol is generic in nature and is intended to provide relevant guidelines for the preparation of nanoparticle dispersions in liquid media by the application of ultrasonic energy (a process referred to here as sonication). It applies specifically to source engineered nanoscale materials (ENM) obtained in dry powder form, but can be extended to the treatment of previously suspended ENMs (e.g., for purposes of re-dispersion).

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CEINT/ NIST Reporting Guidelines for the Prepartion of Aqueous Nanoparticle Dispersions from Dry Nanomaterials, Version 2.1

This document provides general guidelines for reporting relevant conditions and critical parameters relating to the dispersion of nanoparticles in liquid media, including the application of ultrasonic energy or other mechanical dispersion methods (section A), and an example presented as a materials and methods section that can be adapted as needed for different nanomaterials (section B). It applies specifically to source engineered nanomaterials (ENM) obtained in dry powder form. This document is subject to future changes as additional information becomes available.

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NCL Detection of Endotoxin Contamination by End Point Chromogenic LAL Assay

This document describes a protocol for a quantitative detection of Gram negative bacterial endotoxin in nanoparticle preparations using an end-point Limulus Amebocyte Lysate (LAL) assay. The protocol is based on QCL-1000 kit insert manufactured by BioWhittaker/Cambrex Corporation (10.1) and the US FDA Guideline “Validation of the LAL test as an end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices” (10.2). Gram negative bacterial endotoxin catalyzes the activation of proenzyme in the Limulus Amebocyte Lysate. The activated

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Mesocosm Shared Facility

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2010
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Intracellular uptake, intergenerational transfer, and toxicity of silver nanoparticles in Caenorhabditis elegans

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2010
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