Salinity-dependent silver nanoparticle uptake and transformation by Atlantic killifish (Fundulus heteroclitus) embryos

TitleSalinity-dependent silver nanoparticle uptake and transformation by Atlantic killifish (Fundulus heteroclitus) embryos
Publication TypeJournal Article
Year of Publication2013
AuthorsAuffan M, Matson CW, Rose J, Arnold M, Proux O, Fayard B, Liu W, Chaurand P, Wiesner MR, Bottero J-Y, Di Giulio RT
JournalNanotoxicology
Pagination1 - 10
Date Published12/2013
ISSN1743-5404
Abstract

We assessed the biodistribution and in situ speciation of sub-lethal concentrations of citrate-coated silver nanoparticles and dissolved silver within Fundulus heteroclitus embryos. Using a thorough physico-chemical characterization, we studied the role of salinity on both uptake and in situ speciation. The Ag uptake or adsorption on the chorion was reduced by 2.3-fold for Ag NPs, and 2.9-fold for AgNO3 in estuarine water (10‰ ASW) compared to deionized water (0‰ ASW). Between 58% and 85% of the silver was localized on/in the chorion and formed patches between 20 and 80 µm. More than a physical barrier, the chorion was found to be a chemically reactive membrane controlling the in situ speciation of silver. A strong complexation of the Cit-Ag NPs with the thiolated groups of proteins or enzymes of the chorion was responsible for the oxidation of 48 ± 5% of the Ag0 into Ag(I)-S species at 0‰ ASW. However, at 10‰ ASW, the presence of Cl− ions at the surface of Ag NPs slow down this oxidation. For the dissolved silver, we observed that in deionized water 69 ± 7% of Ag+ taken up by the chorion was complexed by the thiolated molecules while the others 30 ± 3% were reduced into Ag0 likely via interaction with the hemiacetal-reducing ends of polysaccharides of the chorion.

DOI10.3109/17435390.2013.869627
Short TitleNanotoxicology