Developmental toxicity and DNA damage from exposure to parking lot runoff retention pond samples in the Japanese Medaka (Oryzias latipes)

TitleDevelopmental toxicity and DNA damage from exposure to parking lot runoff retention pond samples in the Japanese Medaka (Oryzias latipes)
Publication TypeJournal Article
Year of Publication2014
AuthorsColton MD, Kwok KWH, Brandon JA, Warren IH, Ryde IT, Cooper EM, Hinton DE, Rittschof D, Meyer JN
JournalMarine Environmental Research
Date Published04/2014
ISSN01411136
Abstract

Parking lot runoff retention ponds (PLRRP) receive significant chemical input, but the biological effects of parking lot runoff are not well understood. We used the Japanese medaka (Oryzias latipes) as a model to study the toxicity of water and sediment samples from a PLRRP in Morehead City, NC. Medaka exposed in ovo to a dilution series of PLRRP water had increased odds of death before hatching, but not teratogenesis or delayed hatching. Next, we adapted a long-amplicon quantitative PCR (LA-QPCR) assay for DNA damage for use with the Japanese medaka. We employed LA-QPCR to test the hypotheses that PLRRP water and sediments would cause nuclear and mitochondrial DNA damage with and without full-spectrum, natural solar radiation. Fluoranthene with and without natural sunlight was a positive control for phototoxic polycyclic aromatic hydrocarbon-induced DNA damage. Fluoranthene exposure did not result in detectable DNA damage by itself, but in combination with sunlight caused significant DNA damage to both genomes. PLRRP samples caused DNA damage to both genomes, and this was not increased by sunlight exposure, suggesting the DNA damage was unlikely the result of PAH phototoxicity. We report for the first time that PLRRP-associated pollutants cause both nuclear and mitochondrial DNA damage, and that fluoranthene-mediated phototoxicity results in similar levels of damage to the nuclear and mitochondrial genomes. These effects may be especially significant in sensitive marine ecosystems.

DOI10.1016/j.marenvres.2014.04.007
Short TitleMarine Environmental Research